PacBio Sequel II | PacBio Sequel I | NextSeq 500 | 10x Chromium | |
Technology | NGS | NGS | NGS | Molecular barcoding |
Platform | Pacific Biosciences | Pacific Biosciences | Illumina | 10x Genomics |
Instrument Supported | SMRT Cell 8M | SMRT Cell 1M |
|
|
Sequencing Mode |
| CLR |
| - |
Sequencing run Time | Up to 30 hrs (per SMRT Cell) | Up to 20 hrs (per SMRT Cell) | 12-30 hrs | - |
Throughput |
| CLR 25 - 50 Kb |
| - |
Read Length |
| CLR 25 - 50 Kb | 75 bp, 2 x 75 bp | - |
Accuracy | > 99.0% | > 90.0% | > 99.9% | - |
Key Applications |
|
|
| Linked-Reads
Chromium Single Cell Gene Expression
|
Sequencing Facility – Overview