Sequencing Facility – Overview

BACK	PacBio Sequel II	PacBio Sequel I	NextSeq 500	10x Chromium
Technology	NGS	NGS	NGS	Molecular barcoding
Platform	Pacific Biosciences	Pacific Biosciences	Illumina	10x Genomics
Instrument Supported	SMRT Cell 8M	SMRT Cell 1M	High Output Flow Cell Mid Output Flow Cell	Linked-Reads Genomics Single cell barcoding
Sequencing Mode	CLR HiFi	CLR	Single-end (SE) Pair-end (PE)	-
Sequencing run Time	Up to 30 hrs (per SMRT Cell)	Up to 20 hrs (per SMRT Cell)	12-30 hrs	-
Throughput	CLR 50 - 100 Gb HiFi 15 - 30 Gb	CLR 25 - 50 Kb	High Output: 60 -120 Gb Mid Output: 30-60 Gb	-
Read Length	HiFi 10-20 Kb CLR 30 -60 Kb	CLR 25 - 50 Kb	75 bp, 2 x 75 bp 150 bp, 2 x 150 bp	-
Accuracy	> 99.0%	> 90.0%	> 99.9%	-
Key Applications	Whole Genome Sequencing (de novo genome assembly, SV, CNV and Expanded repeat-detection) RNA Sequencing Targeted Sequencing Complex Populations Epigenetics	Whole Genome Sequencing (de novo genome assembly, SV, CNV and Expanded repeat-detection) RNA Sequencing Targeted Sequencing Complex Populations Epigenetics	Whole Exome Sequencing Targeted Gene Sequencing (amplicon, gene panel) Whole-Transcriptiome Sequencing	Linked-Reads Whole Genome Sequencing (de novo genome assembly, SV and CNA detection) Haplotype phasing Complex Populations Chromium Single Cell Gene Expression Cell Characterization Gene Expression Profiling