Sequencing Facility – Overview

BACK	PacBio Revio	PromethION 24	NextSeq 2000	DNBSEQ-G99
Technology			NGS	NGS
Platform	Pacific Biosciences	Oxford Nanopore Technologies	Illumina	MGI
Instrument Supported	25 million ZMWs (per SMRT Cell) 4 stages (4 SMRT Cells)	Up to 24 Flow Cell	Flow Cell P1 Flow Cell P2 Flow Cell P3	FCL Flow Cell
Sequencing Mode	HiFi reads	Short read (200 bp) Long read (N50 up to 50 Kb) Ultra Long read (N50 > 50 Kb)	Single-end (SE) Pair-end (PE)	Single-end (SE) Pair-end (PE)
Sequencing run Time	24 hrs (4 SMRT Cells)	Up to 72 hrs (per Flow Cell)	11 - 48 hrs	5 - 30 hrs
Throughput	Up to 90 Gb (per SMRT Cell)	Up to 100 Gb	30 - 360 Gb	8 - 96 Gb
Read Length	15 - 20 Kb	25 - 50 Kb	50 bp SE, PE 100 bp PE 150 bp PE	100 bp SE/ 50 bp PE 150 bp PE, 300 bp PE 400 bp SE
Accuracy	> 99.9%	> 99.0%	≥ 85% of bases higher than Q30	≥ 75% of bases higher than Q30
Key Applications	Whole Genome Sequencing (de novo genome assembly, SV, CNV, Expanded repeat detection, 5mC methylation and Phasing) RNA Sequencing Targeted Sequencing Complex Populations Epigenetics	Whole Genome Sequencing (de novo genome assembly T2T genome assembly, SV, CNV, Expanded repeat detection 5mC methylation and Phasing) RNA Sequencing Targeted Sequencing Complex Populations Epigenetics	Whole Genome Sequencing (de novo genome assembly, SV, CNV) Whole Exome Sequencing Targeted Gene Sequencing Whole Transcriptome Sequencing	Small Genome Sequencing Whole Exome Sequencing Targeted Gene Sequencing Whole Transcriptome Sequencing